ReliaPrep™ Large Volume gDNA Purification System System Overview Protocol Overview Walk-Away Automation Robust Implementation Scalable Purification Purification Performance Summary High-throughput gDNA isolation from 3ml-10ml blood

ReliaPrep™ Large Volume gDNA Purification System High-throughput gDNA isolation from 3ml-10ml blood System Overview Protocol Overview Walk-Away Automation High-throughput gDNA isolation from3ml-10ml blood Robust Implementation Scalable Purification Purification Performance Summary Larger View • 96 samples processed in ≤8 hours, automated • Robust, precipitation-free protocol • Pure gDNA ready for demanding applications

ReliaPrep™ Large Volume gDNA Purification System System Overview Protocol Overview No user intervention needed once the instrument is loaded Walk-Away Automation Robust Implementation Scalable Purification Purification Performance Blood volume detected Capture and Elute pure release washes DNA Larger View • Load up to 96 samples, add reagents • Start the method • Return in 8 hours to pure gDNA Larger View

ReliaPrep™ Large Volume gDNA Purification System System Overview Protocol Overview Walk-Away Automation Co-design of instrument, accessories and reagents ensures optimal processing Combination of liquid handling and magnetic particle processing Robust Implementation Scalable Purification Purification Performance Hamilton MICROLAB® Star Plus Larger View Purification from up to 96 10ml blood samples Sample processing complete in less than 8 hours Automated reagent delivery and detection of reagent levels No manual intervention

ReliaPrep™ Large Volume gDNA Purification System System Overview Protocol Overview Walk-Away Automation The ReliaPrep™ LV 32 HSM combines a heater, shaker and a magnet for 32 samples in 50ml tubes Robust Implementation Scalable Purification Sample tubes never need to be moved! Purification Performance • Reduces chances of instrument failure • Simplifies sample tracking • Allows efficient use of plastics and reagent Larger View Hamilton Air displacement pipetting with 5ml re-rackable, disposable tips increases speed and decreases plastics usage. Larger View

ReliaPrep™ Large Volume gDNA Purification System System Overview Scalable purification Protocol Overview Walk-Away Automation STAR plus liquid level sensing detects volume of each sample. Robust Implementation Scalable Purification Purification Performance Larger View ReliaPrep™ purification chemistry is scalable • 3ml to 10ml input volume VENUS one Instrument Control Software scales each individual well • provides optimal performance • reduces reagent waste

ReliaPrep™ Large Volume gDNA Purification System Purification Performance System Overview Protocol Overview Walk-Away Automation Robust Implementation Scalable Purification Purification Performance Larger View and Details Larger View and Details Absorbance Ratio

ReliaPrep™ Large Volume gDNA Purification System System Overview Protocol Overview Walk-Away Automation Robust Implementation Scalable Purification Purification Performance Larger View Fully automated, walk-away purification protocol Scalable purification of gDNA from 3ml to 10ml blood samples Purification of 96 samples in less than 8 hours Robust instrumentation resulting in low purification failure rates Efficient gDNA recovery from challenging samples Pure gDNA ready for storage or downstream assays Promega Corporation • 2800 Woods Hollow Road • Madison, WI 53711-5399 USA • Telephone 608-274-4330...